Fig. 3

ISH of the genes suggested to be expressed in the proliferating cells in the pupal MBs. (A-C) The results of ISH of SoxN (A), optix (B), and asense (C) in the MBs of pupae at P-lKC stage, P-mKC stage, P-sKC stage, and P7 pupal stage, respectively. The thin and bold black dashed lines indicate the calyces and areas of KC somata, respectively. (D-E) The results of EdU detection and ISH of SoxN (D), optix (E), and asense (F) using the pupae with MB proliferating cells injected with EdU 2 h before dissection. From left to right panels, the EdU signals, ISH signals, and schematic diagrams of EdU and ISH signals are shown. The white dashed lines in ISH images indicate the region where EdU signals were detected. The intensity of the purple color in schematic diagrams represents the differences in the intensity of ISH signals. Black and gray arrowheads indicate strong and weak ISH signals, respectively. The images in panels A−C include both lateral and medial calyces of the pupal MB, while images and schematics in panels D−F include only one calyx of the pupal MB. Ca, a calyx in the MB. Scale bar; 100 μm.