Fig. 4

ISH of opa in the pupal MBs. (A) The results of ISH of opa in the MBs of pupae at P-lKC stage, P-mKC stage, P-sKC stage, and P7 pupal stage, respectively. The thin and bold black dashed lines indicate the calyces and areas of KC somata, respectively. (B) The results of EdU detection and ISH of opa using the pupae with MB proliferating cells injected with EdU 2 h before dissection. From left to right panels, the EdU signals, ISH signals, and the schematic diagram of EdU and ISH signals are shown. The white dashed line in the ISH image indicates the region where EdU signals were detected. The intensity of the purple color in the schematic diagram represents the differences in the intensity of ISH signals. Black and gray arrowheads indicate strong and weak ISH signals, respectively. Ca, a calyx in the MB. Scale bar; 100 μm.