Fig. 12

Immunohistochemistry (IHC) analysis of TNFSF15 gene expression differences between younger and older groups in the hospital cohort via IHC. (A) and (B) display IHC staining results for the younger and older groups, respectively (scale bar: 500 \(\mu\)m upper panel, 50 \(\mu\)m lower panel). (C) IHC scores quantification between the two groups using the Mann–Whitney U-test, demonstrating significantly higher TNFSF15 expression in younger ccRCC patients (P = 0.0001).