Table 2 ROC analysis of selected EV cargo proteins correlated highly with sepsis diagnosis.

From: Proteomic profiling of plasma extracellular vesicles identifies signatures of innate immunity, coagulation, and endothelial activation in septic patients

Gene name

Gene description

AUC

Z score

References

Complement and Coagulation Cascade

    

Upregulation

    

ITGB2

Integrin beta-2

0.96

1.44

23

VWF

von Willebrand factor

0.95

1.96

24

ITIH4

Inter-alpha-trypsin inhibitor heavy chain H4

0.94

1.39

28

SERPINE1

Plasminogen activator inhibitor 1 (PAI-1)

0.91

1.32

63

SAA1

Serum amyloid A-1 protein

0.93

2.68

64

SERPINA1

Alpha-1-antitrypsin

0.86

1.92

38

Downregulation

    

PF4

Platelet factor 4

0.97

-2.77

65

Immune responses

    

Upregulation

    

ARF5

ADP-ribosylation factor 5

1

1.40

29

GLIPR2

Golgi-associated plant pathogenesis-related protein 1

1

1.79

30

S1PR4

Sphingosine 1-phosphate receptor 4

0.99

1.45

31

SPN

Leukosialin

0.97

1.58

33

CXCR2

C-X-C chemokine receptor type 2

0.92

1.65

66

Downregulation

    

HGFAC

Hepatocyte growth factor activator

1

-2.09

34

HPR

Haptoglobin-related protein

0.98

− 3.32

35

Endothelial activation

    

Upregulation

    

DEFA1

Neutrophil defensin 1

0.99

2.04

36

DEFA3

Neutrophil defensin 3

0.99

2.01

36

S100A9

Protein S100-A9

0.99

1.52

37

S100A8

Protein S100-A8

0.96

1.56

37

CDC42

Cell division control protein 42 homolog

0.86

0.81

39

Downregulation

    

TNN

Tenascin-N

0.97

-2.71

67

Metabolism

    

Upregulation

    

H4C1

Histone H4

0.99

2.64

44

MAPK4

Mitogen-activated protein kinase 4

0.97

2.58

40

SLC16A3

Monocarboxylate transporter 4

0.97

1.97

41

CP

Ceruloplasmin

0.96

1.51

42

H3C1

Histone H3.1

0.95

2.15

44

Downregulation

    

APOL1

Apolipoprotein L1

0.99

-3.16

45

  1. The ROC analysis was performed to estimate the discriminating power between the sepsis and control groups for the selected driving proteins in the M3, M4, M5, M6, and M7 modules overlapped with differentially expressed proteins in sepsis with q ≤ 0.05. Among the proteins with AUC ≥ 0.8, the top 5 to 6 in the highest Z score (upregulation) or one or two of the lowest Z score (downregulation) were selected, which represent intense discrimination and most fold changes. The values in parentheses indicate the lower and upper AUC values within the 95% confidence interval of the repeated AUC analyses conducted by 500 bootstrappings.
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