Fig. 3

(A) mRNA expression of TRPV6 in duodenal tissue from 3-month-old male ICR/Mlac-hydro mice (Hydro) and wild-type (WT) mice (n = 5). (B) Representative immunohistochemistry photomicrographs of TRPV6 protein in WT and Mlac-hydro mice. Positive red-brown signals of TRPV6 protein are predominantly localized at the apical membrane and within the cytoplasm of villous absorptive epithelial cells. Scale bars, 20 μm. (C) mRNA expression of TRPV5, PMCA_1b, and NCX1 in kidney tissue from 3-month-old male Hydro and WT mice (n = 5). *P < 0.05, **P < 0.01 vs. age-matched WT control group. TRPV5, transient receptor potential cation channel subfamily V member 5; TRPV6, transient receptor potential cation channel subfamily V member 6; PMCA_1b, plasma membrane Ca²⁺-ATPase 1b; NCX1, Na⁺/Ca²⁺ exchanger 1.