Fig. 8
(A) Representative photomicrographs of proximal tibial metaphysis of 6-month-old male ICR/Mlac-hydro mice (Hydro) and wild-type mice (WT) stained with Goldner’s trichrome. Scale bars, 1 mm. Epiphyseal plate (Ep), bone trabeculae (arrows), and bone marrow (Ma) are identified. Mineralized bone matrix, erythrocytes, and cytoplasm are stained green, orange, and red, respectively. (B–H) Microstructural analysis of proximal tibial metaphysis in 6-month-old male Hydro and WT mice determined by static bone histomorphometry. (B) trabecular bone volume normalized by tissue volume (BV/TV), (C) trabecular thickness (Tb.Th), (D) trabecular separation (Tb.Sp), (E) trabecular number (Tb.N), (F) osteoblast surface (Ob.S) normalized by bone surface (BS), (G) osteoclast surface (Oc.S) normalized by BS, (H) active erosion surface (aES) normalized by BS. (I–L) Bone microstructural analysis by dynamic bone histomorphometry. (I) double-labeled surface (dLS) normalized by BS, (J) mineralizing surface (MS) normalized by BS, (K) mineral apposition rate (MAR), (L) bone formation rate (BFR) normalized by BS. n = 7; *P < 0.05, **P < 0.01 vs. WT control group.
