Fig. 1

Sex differences of vaccine against Mycobacterium tuberculosis (Mtb) stringent response protein, Rel_Mtb. (A) Summary of experimental plan as performed by Karanika, et al.¹⁰ In brief, C57Bl/6 mice were aerosol infected with 100 bacilli of Mtb strain H37Rv. 28 days later, mice received daily isoniazid (INH) doses at 10 mg/kg by oral gavage. Mice were vaccinated by intramuscular electroporation three times at one week intervals with Rel or MIP-3α-Rel. At week 10 post treatment initiation, lungs were harvested, homogenized, plated at serial ten-fold dilutions on selective agar, and, after growth, colony forming units (CFU) were calculated. Illustration made using Biorender.com (B) Differences in lung bacterial load 10 weeks post treatment initiation of Mtb-infected mice receiving INH alone, INH+ Rel vaccine, and INH + MIP-3α-Rel vaccine from previously published dataset¹⁰, newly stratified by sex of mice. *p < 0.05, **p < 0.01, ****p < 0.0001.