Fig. 2 | Scientific Reports

Fig. 2

From: MIP-3α-antigen fusion DNA vaccine enhances sex differences in tuberculosis model and alters dendritic cell activity early post vaccination

Fig. 2

MIP-3α-EαGFP Model. (A) Map of expressed region of vaccine plasmid with regions and cloning sites labeled. (B) Confirmations of transfection-grade plasmid preps to show DNA purity and supercoiling (C) EαGFP and MIP-3α-EαGFP vaccines verified in vitro by transfection of HEK293T-cells. Cell media was analyzed by semi-denaturing gel followed by blotting and GFP visualization under UV excitation and a green filter (D) Model of system. Secreted vaccine protein will be targeted to iDCs via interaction between MIP-3α and CCR6. Internalization can be tracked by GFP and antigen presentation by Y-Ae antibody designed to interact with I-Ab MHC-II loaded with Eα peptide. (E) Schedule of manuscript experiments: Vaccines administered into mouse gastrocnemius muscle with electroporation. Draining popliteal nodes were harvested two to three days later. Illustrations made using Biorender.com.

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