Fig. 3 | Scientific Reports

Fig. 3

From: MIP-3α-antigen fusion DNA vaccine enhances sex differences in tuberculosis model and alters dendritic cell activity early post vaccination

Fig. 3

Sex and MIP-3α differences of antigen presentation. Eα peptide, inherent in our reporter vaccines as described in Fig. 2, has a known antibody (Y-Ae) that binds to the processed peptide in complex with I-Ab MHC-II on C57Bl/6 mouse APCs, allowing for identification of processed vaccine antigen. Two days post immunization, draining popliteal nodes were harvested, processed, and analyzed by flow cytometry. (A) Antigen presenting cells (APCs) as defined by CD3-CD19-CD11c+ MHCII+ shown as percentage positive for Y-Ae signal. Panels B-D show overall lymph node populations of APCs, including Langerhans cells (B); CD207+F4/80+), and cDCs (CD207-F4/80−) divided into CD8+ (C) and CD11b+ (D) populations. Panels (E–G) define the Y-Ae+ proportions of those APC subsets. Data are representative of two independent experiments, n = 6–8. *p < 0.05, ns, not significant, trending p-values labeled.

Back to article page