Fig. 4

Sex and MIP-3α differences of APC activation within Y-Ae positive populations. Two days post immunization, draining popliteal nodes were harvested, processed, and analyzed by flow cytometry. Langerhans (A, B), CD11b+ cDC (C, D), and CD8+ cDC (E, F) cell populations were analyzed for surface expression levels of CD80 (A, C, E) and MHCII (B, D, F) by comparing mean fluorescence intensity (MFI) measures. Data are representative of two independent experiments, n = 6–8. *p < 0.05, **p < 0.01, trending p-values labeled.