Fig. 3 | Scientific Reports

Fig. 3

From: Evaluation of variability in cell-free DNA extraction efficiency from plasma and urine and spike-in normalization

Fig. 3

Nested ANOVA results from urine samples with adjustment for extraction efficiency and/or urinary creatinine. The variance component estimates are shown for the complete model of the urine long setup (a) with the nested factors distinguished by colour. Inter-individual (b) as well as inter-extraction (c) variance component estimates are shown for the urine long setup. The graphs are separated by extraction method and the analyses for cp/mL unadjusted, and adjusted for creatinine (cpml/UCr), extraction efficiency (cpml/EE) or both (cpml/Ucr/EE) are depicted. Total variance component estimates for the urine combination setup are shown in (d) and the estimates for the inter-individual (e) as well as inter-extraction (f) variability are shown separately. Only samples extracted with Qseph are shown for the urine combination setup with the respective extraction efficiency (EE) and/or urinary creatinine (UCr) adjustments.

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