Table 1 Bacterial strains used in this study.

From: Dissecting the contribution of Kup and KimA to Enterococcus faecalis potassium homeostasis

Strains 

Relevant properties or genotype

Reference or source

E. faecalis

 

JH2-2

RifR, FusR, plasmid-free wild type strain

25

KimA+

ΔktrAΔkup JH2-2 derived

6

Kup+

ΔktrAΔkup JH2-2 derived

6

pTLGR.JH2-2

JH2-2 carrying fluorescent reporter plasmid pTLGR

26

pKupL.JH2-2

JH2-2 carrying fluorescent reporter plasmid pKupL

19

pKimA.JH2-2

JH2-2 carrying fluorescent reporter plasmid pKimA

This study

E. coli

 

DH5-α

F ϕ80d/lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rκ, m+ κ) phoA supE44 λ- thi-1 gyrA96 relA1

27

pTLGR.DH5-α

DH5-α carrying fluorescent reporter plasmid pTLGR

26

pKupL.DH5-α

DH5-α carrying fluorescent reporter plasmid pKupL

19

pKimA.DH5-α

DH5-α carrying fluorescent reporter plasmid pKimA

This study