Table 1 Bacterial strains used in this study.
From: Dissecting the contribution of Kup and KimA to Enterococcus faecalis potassium homeostasis
Strains | Relevant properties or genotype | Reference or source |
|---|---|---|
E. faecalis | ||
JH2-2 | RifR, FusR, plasmid-free wild type strain | |
KimA+ | ΔktrAΔkup JH2-2 derived | |
Kup+ | ΔktrAΔkup JH2-2 derived | |
pTLGR.JH2-2 | JH2-2 carrying fluorescent reporter plasmid pTLGR | |
pKupL.JH2-2 | JH2-2 carrying fluorescent reporter plasmid pKupL | |
pKimA.JH2-2 | JH2-2 carrying fluorescent reporter plasmid pKimA | This study |
E. coli | ||
DH5-α | F− ϕ80d/lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (r−κ, m+ κ) phoA supE44 λ- thi-1 gyrA96 relA1 | |
pTLGR.DH5-α | DH5-α carrying fluorescent reporter plasmid pTLGR | |
pKupL.DH5-α | DH5-α carrying fluorescent reporter plasmid pKupL | |
pKimA.DH5-α | DH5-α carrying fluorescent reporter plasmid pKimA | This study |