Table 4 Correlation between various growth criteria and enzymes activity with different calcium salts of Bacillus subtilis grown for 5 days at successively increasing levels of Time.

	Calcium salts		O.D	Protein	Ammonia content	Urease activity	Nitrogenase activity
Time	Ac	0.50	0.41667	0.886885*	0.532895	− 0.264633	− 0.266502
		1.00	0.52536	0.919315**	0.379146	− 0.300228	0.740715
		1.50	0.28686	0.935375**	0.390579	− 0.297238	0.799242
		2.00	0.08724	0.955662**	0.592404	− 0.243035	0.835985*
	Cit	0.23	− 0.70081	0.880513*	0.429939	− 0.505793	0.948661**
		0.46	0.37485	0.909954*	0.766855	− 0.306067	0.886985*
		0.69	0.08480	0.866760*	0.253276	− 0.431807	0.844762*
		0.95	− 0.03144	0.943370**	0.508702	− 0.381078	0.913322*
	Cl	5.00	0.02353	0.846088*	0.610423	− 0.231668	0.700877
		10.0	0.38292	0.899979*	0.560328	− 0.250310	0.927218*
		15.0	0.23224	0.877540*	0.463238	− 0.275708	0.822274**
		20.0	− 0.13667	0.902228*	0.526992	− 0.228879	0.923089**
	Nit	5.00	− 0.02585	0.772414	0.118963	− 0.365693	0.897497*
		10.0	0.24491	0.838640*	0.558867	− 0.249678	0.874564**
		15.0	− 0.19642	0.883894**	0.522130	− 0.261994	0.831261*
		20.0	0.06764	0.924655**	0.509695	− 0.250989	0.826933*

The positive sign (+) indicates positive correlations and the negative sign (−) indicates negative correlations while one star (*) indicates weak correlation value (< 0.7) and two stars (**) indicate strong correlation (≥ 0.7); no star indicates no correlation.

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