Fig. 2
SFRP5 inhibited Wnt/β-catenin signalling in DKD models. (A) Protein expression of SFRP5 was measured via Western blotting using RTECs after stimulation with high glucose (HG, 30 mM) for 48 h (n = 3). (B) Protein expression of SFRP5 was measured via Western blotting using DKD renal tissue from mice (n = 6); (C) mRNA expression of SFRP5 was measured in HG-stimulated RTECs (n = 3) and DKD renal tissues (n = 6). (D) IHC was used to assess the expression of SFRP5 in DKD renal tissue from mice, and the percent positive area was scored using ImageJ. (E) Protein expression of E-cadherin and Wnt/β-catenin pathway-related factors was measured using RTECs after stimulation with HG (n = 3). The data are expressed as means ± SDs. DKD, diabetic kidney disease; RTECs, renal tubular epithelial cells. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.000 vs. control.
