Fig. 1

Downregulation of miR-338-3p expression in hepatic CSCs. (A) Primary HCC cells (n = 35) were analyzed for their miR-338-3p and CD133 expression levels using RT-PCR. Spearman’s correlation analysis was performed after data were standardized to β-actin as △Ct. (B) Using RT-PCR, we analyzed the relationship between miR-338-3p expression and CD90 expression in primary HCC cells (n = 35). Spearman’s correlation analysis was performed after data were standardized to β-actin as △Ct. (C) We used RT-PCR to examine miR-338-3p expression in CD133 + and CD133- HCC cells, accordingly. (D) Using RT-PCR, we evaluated miR-338-3p expression between CD90⁺ HCC cells and CD90⁻ HCC cells. (E) RT-PCR was utilized to examine miR-338-3p expression in HCC spheroids and adherent cells. (F) RT-PCR measured miR-338-3p expression in HCC spheroids collected in serial passages. Error bars shows mean ± SD derived from n = 3 independent experiments, *p < 0.05.