Fig. 2

miR-338-3p overexpression attenuates hepatic CSCs self-renewal and tumor development. (A) miR-338-3p overexpression virus was used to infect Huh7 and Hep3B cells, and their effects were then evaluated by RT-PCR. (B, C) Comparison of miR-338-3p overexpression cells and control HCC cells regarding CD133 and CD90 expression using RT-PCR. (D) Evaluation of miR-338-3p overexpressing cells and control HCC cells’ ability to form spheroids. Representative images of spheres are shown. (E) In vitro LDA comparing the number of liver CSCs in cells with overexpressed miR-338-3p and control HCC cells. (F) miR-338-3p overexpressing cells and control HCC cells were subjected to an in vivo LDA. After eight weeks, tumors from each group (n=) were analyzed. Error bars shows mean ± SD derived from n = 3 independent experiments, *p < 0.05.