Fig. 4

SIAH2-AS1 knockdown alters EMT and Wnt/β-catenin signaling pathway. (A) Western blot analysis showed that SIAH2-AS1 knockdown reduced the expression of β-catenin, c-myc, and cyclin D1. (B) Western blot analysis showed that SIAH2-AS1 knockdown increased E-cadherin expression, while decreased the expression of Vimentin and N-cadherin. (C-E) The relative expression statistics of β-catenin, cyclin D1 and c-myc in MDA-MB-231 cell. (F-H) The relative expression statistics of β-catenin, cyclin D1 and c-myc in MDA-MB-486 cell. (I-K) The relative expression statistics of E-cadherin, N-cadherin and Vimentin in MDA-MB-231 cell. (L-N) The relative expression statistics of E-cadherin, N-cadherin and Vimentin in MDA-MB-468 cell. (O-Q) Relative expression statistics of Zeb-1, Zeb-2 and Snail mRNA.