Fig. 4
From: Use of bovine serum albumin might impair immunofluorescence signal in thick tissue samples
Immunofluorescence staining of thick murine lymph node tissue samples processed with and without the protein blocking step prior to incubation with primary Abs. (a) immunolabeling of murine lymph node against podoplanin (marker of fibroblastic reticular cells and lymphatic endothelial cells) and CD4 followed by secondary antibodies conjugated with AF-555 and AF-647, respectively. ROI is zoomed in lower panels. (b) mean SBR values of used fluorophores across 20–125 μm imaging range, n = 11–12 per group and (c) SBR values pooled from 20 to 120 μm imaging range, one-way ANOVA with Dunn’s multiple comparison test, n = 11–12 per group. BSA, bovine serum albumin, NGS, normal goat serum. Scale bar, 400 μm and 50 μm in zoomed areas.
