Table 2 Analysis of the consistency of the Sialidase treatment and interference exclusion methods.

From: Polyethylene glycol precipitation is a useful method for excluding the interference of unexplained elevated CA 19-9

Sialidase treatment (N = 342)

PEG method (N = 342)

HBR method (N = 342)

DIL method (N = 342)

RF method (N = 342)

Negative

Positive

Negative

Positive

Negative

Positive

Negative

Positive

Negative

265

255 (96.2%)

10 (3.8%)

258 (97.4%)

7 (2.6%)

253 (95.5%)

12 (4.5%)

255 (96.2%)

10 (3.8%)

Positive

77

0 (0%)

77 (100%)

38 (49.4%)

39 (50.6%)

56 (72.3%)

21 (27.3%)

69 (89.6%)

8 (10.4%)

Cohen’s Kappa coefficient

0.920

0.560

0.285

0.091

  1. Negative sialidase treatment indicates no interference and the Positive sialidase treatment indicates interference; Positive for PEG precipitation: a recovery of serum CA19-9 was less than 37.9%; Positive for HBR treatment: a recovery of serum CA19-9 was less than 47.4%; Positive for the gradient dilution assay: the average recovery rate exceeded the range of 100 ± 15%; Positive for RF assay: the test result was > 20 IU/mL.