Fig. 5
Results of the ASL analysis. (A) Pictures of a transwell with ASL covering the apical surface of the ALI-cultured HPAEpiCs. ASL was not observed on ALI day 7, but was observed on ALI day 14. (B,C) Concentrations of phosphatidylcholine (B) and SP-D (C) in the ASL. The results are expressed as the mean and standard deviation of three independent experiments (five transwell inserts per experiment). Not significant, p > 0.05, versus ALI day 14 by Dunnett’s multiple comparison test. (D) Comparison of the lipidomic data with in vivo values in the literature39. ASL collected on ALI day 28 was analyzed. The results for phosphatidylcholine, phosphatidylglycerol, and phosphatidylinositol are summarized. One pooled sample, derived from 30 transwell inserts prepared in two independent experiments, was used for the lipidomic analysis.
