Table 3 Major proteins detected in the ASL and their exclusive spectrum counts.

From: Comprehensive characterization of human alveolar epithelial cells cultured for 28 days at the air-liquid interface

 

Identified proteins

Accession number

Alternate ID

Molecular weight

Exclusive spectrum count

1

Pulmonary surfactant-associated protein B

P07988

SFTPB

42 kDa

192

2

Complement C3

P01024

C3

187 kDa

163

3

Fibronectin

P02751

FN1

272 kDa

132

4

Complement factor H

P08603

CFH

139 kDa

131

5

Basement membrane-specific heparan sulfate proteoglycan core protein

P98160

HSPG2

469 kDa

88

6

Complement factor I

P05156

CFI

66 kDa

77

7

EGF-containing fibulin-like extracellular matrix protein 1

Q12805

EFEMP1

55 kDa

77

8

Cluster of Actin, cytoplasmic 1

P60709

ACTB

42 kDa

76

9

Polymeric immunoglobulin receptor

P01833

PIGR

83 kDa

76

10

Apolipoprotein E

P02649

APOE

36 kDa

70

11

Alpha-1-antitrypsin

P01009

SERPINA1

47 kDa

67

12

Carboxypeptidase M

P14384

CPM

51 kDa

67

13

Pro-cathepsin H

P09668

CTSH

37 kDa

67

14

Keratin, type II cytoskeletal 8

P05787

KRT8

54 kDa

55

15

Sulfhydryl oxidase 1

O00391

QSOX1

83 kDa

58

16

Ceruloplasmin

P00450

CP

122 kDa

57

17

Acid ceramidase

Q13510

ASAH1

45 kDa

55

18

NPC intracellular cholesterol transporter 2

P61916

NPC2

17 kDa

47

19

Lysosomal alpha-glucosidase

P10253

GAA

105 kDa

47

20

Dipeptidyl peptidase 4

P27487

DPP4

88 kDa

47

  1. The protein identifications were filtered for a minimum of two peptides at 95% confidence and 99% protein confidence.