Fig. 4
From: Unraveling chain specific ubiquitination in cells using tandem ubiquitin binding entities
Analysis of K63 polyubiquitination of endogenous RIPK2. THP1 cells were pre-treated with DMSO (vehicle control) or indicated doses Ponatinib for 30 min followed by treatment with water (control) or 200 ng/ml L18-MDP for 30 min. Cell lysates were subjected to pull downs using pan-selective TUBE (a), K63-selective TUBE (b) or K48-TUBE (c). 30 µg whole cell lysate (d) was used detect RIPK2 expression. Pull down samples and whole cell lysates were immunoblotted with anti-RIPK2 antibody. GAPDH was used as loading control for whole cell lysate. Original western blot images are presented in Supplementary Fig. 4.
