Fig. 2

Alprazolam induced anterograde amnesia is not state dependent. (A,B) Alprazolam treated mice showed significantly greater freezing compared to saline controls on average and across time. (n = 15–25 per group) (C) Alternate timeline 1. Mice injected with saline or alprazolam (1 mg/kg) before both training and re-exposure. Freezing was comparable between alprazolam and saline treated mice during re-exposure. (n = 4–9 mice per group) (D) Alternate timeline 2. Mice injected with saline or alprazolam (1 mg/kg) before training and saline or alprazolam (0.25 mg/kg) before re-exposure. Freezing was comparable between alprazolam and saline treated mice during re-exposure. (n = 5 mice per group) (E) Alternate timeline 3. Mice injected with saline or alprazolam (1 mg/kg) before training and all mice received a saline injection before re-exposure. Alprazolam treated mice show significantly less freezing during re-exposure. (n = 5–11 mice per group). Error bars represent ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; min, minute; Alp, alprazolam; mg, milligram; kg, kilogram; sal, saline; CFC, contextual fear conditioning.