Fig. 4

Alprazolam alters ventral hippocampal ensembles in female but not male mice. (A,B) Alprazolam and saline treated male mice show comparable encoding and retrieval ensemble activation in the ventral hippocampus compared to saline treated controls. (C,D) Alprazolam and saline treated male mice show comparable engram activation in the ventral hippocampus compared to saline controls. (E,F) Representative images of tagged ensembles in saline and alprazolam treated male mice. (G) Alprazolam and saline treated female mice show comparable encoding ensemble activation in the ventral hippocampus. (H) Alprazolam treated female mice show significantly less retrieval ensemble activation in the ventral CA1 compared to saline treated controls. (I,J) Alprazolam treated females do not show differences in engram activation in the ventral hippocampus. (n = 5 mice per group). Error bars represent ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; vDG, ventral dentate gyrus; vCA3, ventral cornu ammonis 3; vCA1, ventral cornu ammonis 1.