Fig. 3 | Scientific Reports

Fig. 3

From: Testing the limits of short-reads metagenomic classifications programs in wastewater treating microbial communities

Fig. 3

Bar plots illustrating the relative abundances of the most abundant misclassified genera across classifications obtained using various programs and parameter settings. The displayed relative abundances account for the correct classification counts (not shown in this plot), thereby representing the total extent of misclassifications. The x-axis represents the classification types: “E” denotes the E-value threshold, “m” indicates the coverage threshold, and “c” represents the confidence level of the classification, depending on the classifier available options. The prefix “contigs” specifies classifications based on contigs rather than individual reads in case of Kaiju and Kraken2. The prefixes “default”, “metalarge” and “custom” refer to the different MEGAHIT assembly settings.

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