Fig. 6
From: Pex3 promotes formation of peroxisome-peroxisome and peroxisome-lipid droplet contact sites
Overexpression of Pex3 leads to morphological changes in lipid droplets and peroxisomes in Drosophila melanogaster. (A, E) Diagrams of the cuts from Drosophila melanogaster that were analyzed. Larval midgut (A) and midgut from adult flies (B) were analyzed. (B–D) Representative images of control cells and strains overexpressing Pex3 in larval gut. Control cells express the GFP-SKL construct to visualize the lumen of the peroxisomes, while cells with overexpressed Pex3 also contain a GFP tag. Lipid droplets were stained with Bodipy and the nucleus with DAPI. Scale bars: 20 μm. Panels C and D show the quantification of the number of LDs (C) and peroxisomes (D). (F–H) Representative images of cells from the adult midgut from control flies and flies overexpressing Pex3. Control cells express the GFP-SKL construct to visualize the lumen of the peroxisomes, while cells with overexpressed Pex3 also contain a GFP tag. Lipid droplets were stained with Bodipy and the nucleus with DAPI. Scale bars: 20 μm. Panels G and H show the quantification of the number of LDs (C) and peroxisomes (D). (I) Representative Images of cells from adult flies midgut overexpressing Pex3-HA. Cells also express YFP-SKL to label the lumen of peroxisomes and lipid droplets were labeled with Bodipy. The enlarged organelles are observed in close proximity, with complementary morphological changes, suggesting the formation of membrane contact sites.
