Fig. 1

Isolation and characterization of primary mouse hepatocytes from the liver of newborn C57 mice. (A) primary mouse hepatocytes morphotype under bright-field microscopy. Scale bar = 200 μm. (B, C) PAS staining of glycogen in primary mouse hepatocytes. Scale bar = 100 μm and 50 μm. (D, E) Immunofluorescence staining for ALB and CK18 (hepatocyte markers) expression in primary mouse hepatocytes. Scale bar = 200 μm and 50 μm.