Fig. 2 | Scientific Reports

Fig. 2

From: The key regulation of LncRNA MALAT during reprogramming of primary mouse hepatocytes into insulin producing cells

Fig. 2

Primary mouse hepatocytes differentiation into IPC. (A) Schematic presentation of the step-wise differentiation protocol used to generate IPCs from primary mouse hepatocytes. (B) Photographs of primary mouse hepatocytes differentiated into IPCs. Scale bar = 400 μm and 200 μm. (C) Identifying PDX1 cells using immunofluorescence staining with PDX1 antibody and expression of β-cell development-related genes. (D) Detection of insulin-producing cells using immunofluorescence staining with insulin and C-peptide antibodies and expression of the insulin gene. (n = 3, **P < 0.01, ***P < 0.001).

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