Table 1 Ingredient and nutrient composition of basal diet and addition of oral vaccine.

From: Surface display of Nocardia seriolae HRP1 on Bacillus subtilis and its application as live vaccine for largemouth bass

Ingredient (%)

Control

B. subtilis

HRP1-B. subtilis

Fish meal

66

66

66

Corn starch

5

5

5

Fish oil

2

2

2

Soybean oil

2.8

2.8

2.8

Soybean lecithin

1

1

1

Monocalcium phosphate

1

1

1

Sodium alginate

4

4

4

Choline chloride

0.4

0.4

0.4

Mineral premixa

1

1

1

Vitamin premixb

1

1

1

α-cellulose

15.8

15.8

15.8

Bacteria Supplementation

0

108 cfu/g B. subtilis

108 cfu/g HRP1-B. subtilis

  1. aMineral premix (g/kg): the mineral premix formula referred to Li et al.(2020a). The details were as follows: NaCl, 363.88; MgSO4·7H2O, 586.67; FeSO4·7H2O, 22.22; A1Cl3·6H2O, 0.67; KI, 0.67; CuSO4·5H2O, 2.22; MnSO4, 4.67; CoCl·6H2O, 0.86; ZnSO4·7H2O, 18.09; Na2SeO3, 0.06.
  2. bVitamin premix (g/kg): the vitamin premix formula referred to Li et al. (2020a). The details were as follows: vitamin A, 2.31; vitamin D3, 2.02; vitamin E, 20.00; vitamin K3, 1.20; vitamin C, 30.00; vitamin B5, 10.87; inositol, 15.00; niacin,14.00; vitamin B6, 3.04; vitamin B2, 3.00; vitamin B1, 3.26; biotin, 0.15; folic acid, 0.60; vitamin B12, 0.02; cellulose, 894.53.