Fig. 1

Murine ischemic model and characterization of E-MSCs. (a) Before and after ischemia induction (b) Morphology of E-MSCs after plating from days 1 to 6 (×40 and ×100 magnifications, respectively). (c) E-MSCs strongly expressed CD29, CD44, CD73, and CD105. The black lines indicate isotype-matched control staining. The blue lines represent cells stained with fluorescent dye-conjugated antibodies. FA, femoral artery; PCFA, proximal caudal femoral artery; SCEA, superficial caudal epigastric artery, HLA, human leukocyte antigen; PE-A, phycoerythrin-conjugated antibodies; SSEA, stage-specific embryonic antigen; TRA, tumor-related antigen.