Fig. 5 | Scientific Reports

Fig. 5

From: Angiogenic and Immunomodulatory effects of embryonic stem cell derived mesenchymal stem cells in a murine model of ischemic hindlimb

Fig. 5

Histological analysis and staining images of muscle tissues. (a–d) Compared to G1, ischemia led to decreased myofiber thickness, increased muscle degeneration, and a tendency to elevate inflammation and fibrosis. On day 21, the E-MSC-treated groups showed significant muscle regeneration and a trend of reduced inflammation and fibrosis compared to G2. However, no differences were seen based on E-MSC doses. The number of myofibers/mm² was measured to illustrate the extent of muscle regeneration. (*, P < 0.05 and **, P < 0.01) (e) Hematoxylin and eosin (H&E) and (f) Masson’s trichrome (MT) staining for ischemic muscle (×200 magnification). In H&E staining, infiltrated inflammatory cells appear dark purple, whereas collagen fibers appear blue after MT staining. (Scale bar: 100 μm).

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