Table 1 Sequences of primers used and quantitative polymerase chain reaction conditions.
Gene | Sequence | |
|---|---|---|
IL-1Β | forward: | 5′-GAT AAC CTG GTG TGT GA-3′ |
reverse: | 5′-GTT CAT CTC GGA GCC TGT AG-3′ | |
IL-6 | forward: | 5′-TCA CAA GTC GGA GGC TTA ATT ACA-3′ |
reverse: | 5′-TGC ACA ACT CTT TTC TCA TTT CCA-3′ | |
TNF-Α | forward: | 5′-CCC TGG CTG CCC TTC AC-3′ |
reverse: | 5′-TCA TTG AGG GCT TCT CTT GTT CT-3′ | |
TGF-Β1 | forward: | 5′-GAG CCC TGG ATA CCA ACT AT-3′ |
reverse: | 5′-AGA CAG AAG TTG GCA TGG TA-3′ | |
ANG-1 | forward: | 5′-GGG GAG GTT GGA CAG TAA TA-3′ |
reverse: | 5′-AGC ATG TAC TGC CTC TGA CT-3′ | |
FGF-2 | forward: | 5′-GCT GGC TTC TAA GTG TGT TA-3′ |
reverse: | 5′-AGT TTA TAC TGC CCA GTT CG-3′ | |
VEGF | forward: | 5′-GGT TTA AAT CCT GGA GCG TT-3′ |
reverse: | 5′-CGT TCG TTT AAC TCA AGC TG-3′ | |
PDGF | forward: | 5′-CAG ATC TCT CGG AAC CTC AT-3′ |
reverse: | 5′-CAC ATT GCG GTT ATT GCA G-3′ | |
GAPDH | forward: | 5′-TGA TGG GTG TGA ACC ACG AG-3′ |
reverse: | 5′-GAT GGC ATG GAC TGT GGT CA-3′ | |
RNA extraction | TRIzol LS Reagent; Invitrogen | |
cDNA synthesis | RevertAid first strand cDNA synthesis kit; Thermo Fisher Scientific | |
PCR reagent | SsoAdvanced Universal SYBR Green Supermix | |
qPCR conditions: | 95 °C, 30 s | |
95 °C, 10 s | × 40 cycles | |
60 °C, 20 s | ||
PCR instrument | CFX384 Touch Real-Time PCR Detection System; Bio-Rad Laboratories | |
