Fig. 4
Combined inhibition of TRAF6 and CsA induced allograft long-term survival in chronic cardiac transplantation rejection model. (A) The murine cardiac heart transplantation model was performed using BALB/C mice as donors and C57BL/6 mice as recipients. CD40-TRAF6 specific antagonist (with or without cyclosporin A) was administered after transplantation (n = 5 for each group). (B) Mean survival times of cardiac allografts in different groups. CD40-TRAF6 inhibitor and CsA-induced long-term allograft survival. CsA vs CsA + CT6i (p = 0.002) (95% CI 0.3750 (0.1086–295) (C) HE staining of cardiac allografts from recipient mice 4 weeks after heart transplantation. (D) Masson staining of cardiac allografts from recipient mice 4 weeks after heart transplantation. (E,F) Infiltrated CD4 + (p = 0.001), CD11b + (p = 0.016) cells in the allografts 4 weeks after heart transplantation. (G,H) FCM assays of CD4 + CD154 + splenocytes (p = 0.001).
