Fig. 5 | Scientific Reports

Fig. 5

From: Mitigating cardiac allograft vasculopathy in a murine model via CD40-TRAF6 blockade and cyclosporin A synergy

Fig. 5

CD40-TRAF6 inhibition affected the immunostimulation function of BMDCs in vitro. (A) The mixed lymphocyte reaction (MLR) was used to assess T-cell proliferation. (B) CCK-8 proliferation analysis of the lymphocyte mixing assay: only high concentrations of CD40-TRAF6 inhibitor (10 uM) affected the OD value (p = 0.045). (C) CFSE-labeled CD4 + T cell (activated by CD3/CD28) proliferation was not hampered by CD40-TRAF6 inhibition (10 uM)(p = 0.287). (D) Proliferation of CFSE-labeled CD4 + T cells (co-cultured in BMDMs) was hampered by CD40-TRAF6 inhibition (10 uM) (p = 0.014).

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