Fig. 6 | Scientific Reports

Fig. 6

From: Mitigating cardiac allograft vasculopathy in a murine model via CD40-TRAF6 blockade and cyclosporin A synergy

Fig. 6

TRAF6 inhibition induced BMDCs developed a less pro-inflammatory phenotype via NF-κB pathway. (A) Macrophages were activated by stimulation in vitro. (B) Immunofluorescence assay of CD68 + Arg-1 + BMDMs. (C) Immunofluorescence assay of CD68 + i-NOS + BMDMs. (D) CT6i decreased CD11b + CD86 + BMDMs in M1 polarization (p = 0.001). Slightly decreased CD11b + CD163 + BMDMs in M2 polarization. (p = 0.002) (E) Transwell assay showing that the migration capacity of BMDMs was hampered by CT6i. (F,G) scRNAseq shows the expression increased pathway in macrophage activation. (H,I) Western blot assay of NF-κB pathway proteins showed that the phosphorylation level of IκBα was decreased.

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