Fig. 9

Bactericidal effect of ME nanostructures monitored using Dead/Live Baclight in (a) E. coli and (b) S. aureus cultured on hPDMS substrate also , including configurations with microscale features such as LAR HPA and LAR gratings, for a duration of 300 min . . The percentage of dead bacteria was calculated every 10 min from fluorescent stained bacteria images. The profiles indicate that MEnanostructures exhibit greater bactericidal effectiveness against S. aureus compared to E. coli. In the case of the hierarchies, the bactericidal effect of ME nanocones was remarkably earlier but fluctuating over time.