Fig. 2

Peptide binding assays. ab, Fluorescence polarization peptide binding assays to Vps4p (a) and Vps4p^∆N (b), across hexamer concentrations 0–33.3µM. c, Fluorescence polarization peptide binding assays to Vps4p^∆N, shown across hexamer concentrations 0–2µM to distinguish the tightest-binding peptides: (VK)₅, (EK)₅, and peptide-8.