Table 2 Primer sequences for qPCR used in this study.
From: PknB and STP as potential targets of luteolin in combating Trueperella pyogenes infections
Gene | Sequence (5ʹ-3ʹ) | Product size (bp) | Temperature (°C) |
|---|---|---|---|
pknB | forward: TCGACTTCTCACCACTGAACAC | 188 | 56.9 |
reverse: CTTTCATCAGTGCAAGCGTGA | |||
stp | forward: TCGTCGTCGGCATCCTGTCC | 112 | 61 |
reverse: TTGTCTGCGTCATTGTGGCTGAG | |||
gapdh | forward: CGGCGAAGAACGAGGACATCAC | 153 | 63.5 |
reverse: GTCGGCAGTGTAGGCGTGAAC |
