Table 3 Primer sequences used in the expression of the His-PKNB and His-STP fusion proteins.
From: PknB and STP as potential targets of luteolin in combating Trueperella pyogenes infections
Gene | Sequence (5ʹ-3ʹ) | Product size (bp) | Temperature (°C) | Restriction site |
|---|---|---|---|---|
pknB | forward: CGGGATCCATGG CTGACGTACCCCGAA | 998 | 56.9 | BamH I |
reverse: TAAAGCGGCCG CTTACGGCCGAACAGATGGCGTA | Hind III | |||
stp | forward: CGGGATCCATGG CTGACGTACCCCGAA | 732 | 61 | BamH I |
reverse: CCCAAGCTTTCGATTG TGTTTGTTCTCTTCAACCG | Hind III |
