Fig. 1

Immunohistochemical (IHC) analyses of MPO⁺ TANs and MPO⁺ non-neutrophils in PT from MBC patients. (A) IHC double staining of CD15 (brown) and MPO (pink) in human breast cancer tissue, where CD15⁺MPO⁻ cells represent TANs/G-MDSCs in an immature/non-activated state, CD15⁺MPO⁺ mature/activated TANs/G-MDSCs, CD15⁻MPO⁺ non-neutrophils (monocyte/macrophages) expressing MPO and CD15⁺ TCs malignant cells expressing CD15. All histological sections were counterstained with H&E. The scale bars shown are representative for all images (20 μm for magnified inserts and 50 μm for full images). (B) Pie chart showing descriptive statistics of amount (%) or tumors with infiltration of any immune cell (IC; grey) (CD15⁺MPO⁻, CD15⁻MPO⁺, CD15⁺MPO⁺), CD15⁺ TCs with simultaneous infiltration of any IC (checkered), CD15⁺ TCs alone (black) or no staining (negative; white). Also see Supplementary Table 2.