Table 6 Benchmarking of the proposed A-SD-HJ-DD-UTFET biosensor against existing POCT biosensing technologies and commercial diagnostic kits for DENV NS1 detection.
Various POCT biosensors/commercial diagnostic tests | Analyte | Sensing method | Advantages | Disadvantages | Refs. |
|---|---|---|---|---|---|
Electrochemical biosensor | Nucleotide sequence | Electrochemical sensing | Miniaturization and Potential multiplexing | Requires complex surface functionalization and target amplification | |
Lateral flow strips | DENV NS1 | Immunochromato-graphic Test (ICT) | Portable and User friendly | Lower sensitivity for certain DENV serotypes | |
Microfluidic Chips | DENV serum & NS1 | Lab-on-chip with microchannels | Compact and low sample requirement | Fabrication complexities | |
Optical genosensor | RNA-DNA | Optical (SPR or Fluorescence) | Effective for DNA-RNA hybridization | Requires a longer time and Expensive instrumentation | |
PanBio dengue early ELISA Kit | DENV NS1 | Enzyme-Linked Immunosorbent Assay | Provides relatively rapid results (~ 2 to 3 h) | Lower Sensitivity, particularly during very early infection | |
Dengue NS1 Ag Strip kit | DENV NS1 | ICT Technique | Higher specificity than some ELISA kits | Lower Sensitivity compared to standard ELISA techniques | |
Omega capture ELISA (IgM assay) | IgM | Immobilized anti-IgM antibody on ELISA plate | Higher specificity for recent infections | Lower sensitivity, Requires Febrile samples (above 5 days) | |
SD Bioline IgG | IgG | Immobilized anti-IgG antibody on lateral flow strip | Moderate sensitivity and specificity, Easy to operate | High cost and not suitable for early detection | |
FET Based Biosensor (A-SD-HJ-DD-UTFET) | DENV NS1 | Dielectric modulation based sensing | Higher sensitivity, Early detection, Low cost, Rapid response and Serotype & Label-free detection | Yet to be commercialized | This work |
