Fig. 7

Anticancer activity of C. amylolyticum type II L-asparaginase in different cell lines after 24 h treatment. (A) Cytotoxicity evaluation in THP-1cell lines; (B) Cytotoxicity evaluation in MOLT-4 cell lines; (C) Cytotoxicity evaluation in MCF-7 cell lines (D) Cytotoxicity evaluation in human adult dermal fibroblast cells; (E): Morphology of THP-1 cells (negative control); (F): Morphology of THP-1 cells after 24 h of purified enzyme treatment; (G): Morphology of MOLT-4 cells (negative control); (H): Morphology of MOLT-4 cells after 24 h of purified enzyme treatment; (I): Morphology of MCF-7 cells (negative control); (J): Morphology of MCF-7 cells after 24 h of purified enzyme treatment; (K): Results of apoptosis assay in MOLT-4 leukemia cells (negative control); (L): Results of apoptosis assay in MOLT-4 cells treated with the purified enzyme at IC50 concentration). In figures (A to D): Error bars indicate the standard deviation from the average enzyme activity. Letters starting from ‘a’ to ‘g’ indicate different levels based on statistical significance (p < 0.05). Figures (F, H, and J) show the cells undergoing apoptosis and are characterized by cellular rounding up, shrinkage, membrane blebbing and loss of cell adhesion. In figure (K and L), the quadrant Q1, Q2, Q3, and Q4 shows the percentage of dead cells, late apoptotic, early apoptotic, and live cells, respectively.