Table 1 Statistical optimization of C. amylolyticum type II L-asparaginase production using Plackett–Burman design.
Std | Run | A | B | C | D | E | F | G | H | J | K | L | L-asparaginase activity (U/ml) | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A (%) | pH | Temp (oC) | Inoculum (%) | Agitation (rpm) | K2HPO4 (%) | NaCl (%) | Peptone (%) | NH4Cl (%) | D1 | D2 | Experimental | Predicted | Residuals | ||
2 | 1 | − 1 | 1 | 1 | − 1 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 1 | 67.01 ± 0.53 | 62 | 5.01 |
11 | 2 | 1 | − 1 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 1 | − 1 | 1 | 130 ± 1.053 | 124.98 | 5.02 |
12 | 3 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | − 1 | 127.9 ± 0.42 | 123.98 | 3.98 |
5 | 4 | − 1 | − 1 | 1 | − 1 | 1 | 1 | − 1 | 1 | 1 | 1 | − 1 | 128.7 ± 0.87 | 126.88 | 1.82 |
7 | 5 | 1 | − 1 | − 1 | − 1 | 1 | − 1 | 1 | 1 | − 1 | 1 | 1 | 118 ± 0.31 | 118.19 | − 0.18 |
10 | 6 | − 1 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 1 | − 1 | 1 | 1 | 138.9 ± 0.76 | 142.16 | − 3.26 |
9 | 7 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 1 | − 1 | 1 | 1 | − 1 | 19.75 ± 1.22 | 24.76 | − 5.01 |
3 | 8 | 1 | − 1 | 1 | 1 | − 1 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 152.9 ± 0.55 | 156.49 | − 3.59 |
4 | 9 | − 1 | 1 | − 1 | 1 | 1 | − 1 | 1 | 1 | 1 | − 1 | − 1 | 69.8 ± 1.22 | 58.98 | 10.82 |
8 | 10 | 1 | 1 | − 1 | − 1 | − 1 | 1 | − 1 | 1 | 1 | − 1 | 1 | 61 ± 0.49 | 66.61 | − 5.61 |
ANOVA table | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Factor | Coefficient estimate | Standardized effect | Contribution (%) | p-value | |||||||||||
Intercept | 98.83 | ||||||||||||||
Model | 0.003 | ||||||||||||||
B-Media pH | − 22.75 | − 45.5067 | 34.77 | 0.0002 | |||||||||||
C-Temperature | 7.38 | 14.76 | 3.658 | 0.0241 | |||||||||||
D-Inoculum | 11.77 | 23.54 | 9.3 | 0.0038 | |||||||||||
G-NaCl | − 15.59 | − 31.1733 | 16.31 | 0.0011 | |||||||||||
H-Peptone | 12.72 | 25.44 | 10.867 | 0.0027 | |||||||||||
J-NH4Cl | − 18.62 | − 37.24 | 23.29 | 0.0005 | |||||||||||
R2 | 0.9821 | ||||||||||||||
Adjusted R2 | 0.9606 | ||||||||||||||
Predicted R2 | 0.8968 | ||||||||||||||
