Fig. 2
Adhesion molecules accumulated at the contact interface of adjacent cells. (A) Time-lapse images of E-cadherin-expressing cells. Box indicates the region where cell membrane fluorescent intensity was measured in (B). Time (h:min) since the start of observation is shown (see Video 2). Bar: 20 µm. (B) Fluorescence intensity profile of cells shown at the top. (Left panel) Cells prior to contact; (middle) cells immediately after contact; (right) cells immediately after detachment. (C) Time-lapse images of IZUMO1-expressing cells (green) and JUNO expressing-cells (magenta). Time (h:min) since the start of observation is shown (see Video 3). Bar: 20 µm. Arrowheads indicate IZUMO1 accumulation at the contact site. (D) Fluorescence intensity profile of IZUMO1 in a cell shown at the top. (E) IZUMO1 accumulation rate for various treatment combinations. Numbers indicate contacted cell pairs observed by live imaging.
