Fig. 2
From: A geminivirus attenuation vector for crop protection using episomal plant gene therapy
A schematic of the attenuation vector strategy for a bipartite begomovirus. The coat protein is replaced with nanoluciferase (NLuc) for quantitative assessment of attenuation for the Target vector. A silencing small RNA siRNA on the attenuation vector avoids self-attenuation due to a re-coding of the viral sequence for the TrAP gene (cmTrAP). Both the circular and linear replicating forms of the A-component are shown to represent the alternative viral replication modes of rolling circle (RCR), and recombinant-dependent replication (RDR).
