Table 3 Comparative percentage reduction for various yield, reproductive, physiological, oxidative and biochemical traits between cold-tolerant and cold-sensitive Cicer accessions under control and cold-stress conditions during second year screening (2022–23).
Traits | Cold tolerant accessions | % reduction | Cold sensitive accessions | % reduction | ||
|---|---|---|---|---|---|---|
Control | Cold stress | Control | Cold stress | |||
Filled pods (FP) | 90 | 68 | 24.44 | 43.0 | 10 | 76.74 |
Number of seeds (NS) | 92 | 72 | 21.74 | 36.0 | 10 | 72.22 |
Total seed weight (TSW) | 8.10 | 4.50 | 44.44 | 2.55 | 0.26 | 89.80 |
Pollen viability (PV) | 97 | 76 | 21.65 | 86.0 | 60 | 30.23 |
Pollen germination (PG) | 93 | 80.3 | 13.66 | 86.0 | 69 | 19.77 |
Pollen load (PL) | 5 | 3.5 | 30.00 | 4.0 | 1.8 | 55.00 |
Ovule viability (OV) | 4.8 | 3.3 | 31.25 | 3.5 | 2.0 | 42.86 |
Electrolyte leakage (EL) | 10.4 | 17.3 | 66.35 | 14.8 | 27.1 | 83.11 |
Relative leaf water content (RLWC) | 84.4 | 80.7 | 4.38 | 80.8 | 70.2 | 13.12 |
Stomatal conductance (SC) | 204.4 | 117.7 | 42.42 | 195.2 | 87.6 | 55.12 |
Chlorophyll content (SPAD) | 43.7 | 36.2 | 17.16 | 36.8 | 22.3 | 39.40 |
Photosynthetic efficiency (PE) | 0.8 | 0.69 | 13.75 | 0.74 | 0.55 | 25.68 |
Carotenoids (CART) | 1.7 | 3.9 | 129.41 | 1.7 | 2.99 | 73.84 |
Proline (PRO) | 16.2 | 29 | 79.01 | 14.9 | 19.6 | 31.54 |
Trehalose (TRE) | 2.7 | 4.4 | 62.96 | 1.8 | 2.3 | 27.78 |
Total sugars (TS) | 51.6 | 75.2 | 45.74 | 35.5 | 44.1 | 24.23 |
Malondialdehyde content (MDA) | 8.3 | 12.7 | 53.01 | 8.4 | 22.7 | 170.24 |
Hydrogen peroxide (H202) | 1.2 | 2.4 | 100.00 | 1.3 | 4 | 207.69 |
Superoxide dismutase (SOD) | 10.7 | 24.4 | 128.04 | 9.8 | 14.6 | 48.98 |
Catalase (CAT) | 2.9 | 5.5 | 89.66 | 2.4 | 3.5 | 45.83 |
Ascorbate peroxidase (APX) | 3.2 | 6.6 | 106.25 | 2.8 | 4.1 | 46.43 |
Glutathione reductase (GR) | 3.5 | 5.8 | 65.71 | 2.1 | 3.1 | 47.62 |
Ascorbate (ASC) | 62.5 | 120.1 | 92.16 | 62.0 | 86.3 | 39.19 |
Glutathione reduced (GSH) | 14.9 | 34.9 | 134.23 | 13.3 | 17.5 | 31.58 |
