Fig. 2

Local analyses of bone cells. (a) Alcohol did not cause differences in the number of osteoclasts after 24 h. (b) An additional hemorrhagic shock led to more osteoclasts after three weeks in young animals in comparison to the Fx group or three weeks. (c) In contrast to sober animals, drunken young Sham and Fx mice showed higher numbers of osteoblasts in the fracture gap three weeks after trauma. For osteocytes (d) no effects of alcohol consumption occurred. (e) More red stained cells, with bone contact, interpreted as osteoclasts in the TRAP staining, appeared in the young sober THFx groups than in the respective Fx animals after three weeks. (f) Significant changes in the numbers of osteoblasts after three weeks: violet, cubic cells arranged in lines with contact to bone, counted as osteoblasts in ImageJ, are marked with crosshairs and yellow numbers. Scale bar 100 μm, magnification 300-fold. * p < 0.05 vs. indicated group, # p < 0.05 vs. respective Sham. (A) n = 3–8, (B) n = 5–6, (C) n = 5–6, (D) n = 5–6 per group.