Fig. 1
From: Corneal safety assessment of germicidal far UV-C radiation
UV-induced DNA damage in the epithelium of reconstructed human cornea (RHCE). (a) DNA damage, as percentage of cells with cyclobutane pyrimidine dimers (CPD, blue) and pyrimidine-pyrimidone (6 − 4) photoproduct (6-4PP, orange) formation, determined by immunohistochemistry, fixed immediately and 24 h after single or multiple UV irradiation (222 nm–150 mJ/cm2, 233 nm–60 mJ/cm2, and broadband UV-B (280–400 nm)–3 mJ/cm2). Different tissue sections were stained separately for each lesion type using lesion-specific antibodies. Pairwise group differences were assessed using one-way ANOVA followed by Bonferroni-corrected post hoc tests. Far UV-C irradiation induces superficial damage in epithelial cells, with no significant repair observed. Following multiple irradiations, CPD damage accumulates. Data represents mean ± SEM (n = 3–4). (b) Representative images of immunohistochemical detection of CPD and 6-4PP positive cells in the epithelium of RHCE. Positive cells are stained in dark red. Scale bar: 100 μm.
