Fig. 5
From: Corneal safety assessment of germicidal far UV-C radiation
UV-induced DNA damage in the (a) epithelium, (b) anterior and posterior stroma of porcine cornea. DNA damage, as percentage of cells with cyclobutane pyrimidine dimers (CPD, blue and green) and pyrimidine-pyrimidone (6 − 4) photoproduct (6-4PP, orange) formation, determined by immunohistochemistry, fixed immediately and 24 h after UV irradiation (233 nm–40 mJ/cm2, 254 nm–40 mJ/cm2 and broadband UV-B (280–400 nm)–3 mJ/cm2). Irradiation at 233 nm induces superficial damage to the epithelium, UV-B causes more extensive damage throughout the epithelium, and 254 nm penetrates into the anterior stroma, inducing the highest level of damage. Data represents mean ± SEM (n = 2). (c) Representative images of immunohistochemical detection of CPD-positive cells in the epithelium of porcine cornea samples. Positive cells are stained in dark red. Scale bar: 100 μm.
