Fig. 1

Workflow of integration of autoradiography with underlying anatomy. After harvesting, the tissues were snap-frozen, and at least 3 sections were adhered to a microscope slide and imaged on the autoradiography device. The same sections were then stained for morphology and subsequently imaged fluorescently (P&H). By combining multiple sections on a microscope slide, the locations of each section, relative to each other, were fixed between autoradiography and morphological imaging. Consequently, there exists a perfect 1:1 (rigid) mapping between the two imaging modalities, which aides creating a near-perfect overlay between the two by increasing the robustness of the coregistration, particularly against rotation and translation imperfections.